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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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Genetics
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JoVE Journal Genetics
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

DOI:
10.3791/64798-v

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08:18 min

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May 12, 2023

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, , , , , , , , , , ,
1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Chapters

  • 00:04Introduction
  • 00:40Setup for the Initial Separation of Trichomes from the Plant Material
  • 02:08Separation of Trichomes from the Plant Material
  • 03:24Separation of Glandular Capitate Stalked and Sessile Trichomes from Other Trichome Types
  • 05:13Passing Trichomes Through Micro-Sieves of Decreasing Pore Size and Collection of the Desired Trichomes
  • 06:14Results: High-Throughput Isolation & Enrichment of Glandular Capitate Stalked & Sessile Trichomes from Cannabis sativa
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

Tags

Keywords: Non-aqueous IsolationEnrichmentGlandular Capitate Stalked TrichomesGlandular Capitate Sessile TrichomesCannabis SativaHigh ThroughputHigh ConcentrationTrichome DensityDry IceLiquid NitrogenMesh SieveExtractionPlant MaterialPlant Processing

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