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JoVE Journal Genetics
Isolamento non acquoso e arricchimento di tricomi ghiandolari e sessili da Cannabis sativa
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Isolamento non acquoso e arricchimento di tricomi ghiandolari e sessili da Cannabis sativa

Article DOI: 10.3791/64798-v • 08:18 min • May 12th, 2023
May 12th, 2023 •
1, 1, 1, 2, 2, 1, 3, 3, 4, 2, 3, 1
1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center

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Viene presentato un protocollo per l'isolamento e l'arricchimento conveniente e ad alto rendimento dei tricomi ghiandolari e sessili da Cannabis sativa. Il protocollo si basa su un'estrazione secca e non tampone di tricomi utilizzando solo azoto liquido, ghiaccio secco e setacci di nylon ed è adatto per l'estrazione dell'RNA e l'analisi trascrittomica.

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Genetica Numero 195
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