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JoVE Journal Neuroscience
Isolering og dyrking av vestibulære og spiralganglion somata fra nyfødte gnagere for patch-klemmeopptak
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Isolering og dyrking av vestibulære og spiralganglion somata fra nyfødte gnagere for patch-klemmeopptak

Article DOI: 10.3791/64908-v • 11:05 min • April 21st, 2023
April 21st, 2023 •
1, 1, 1, 1, 1, 1
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program, University of Southern California

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Presentert her er metoder som gir detaljerte instruksjoner for dissekering, dissosiering, dyrking og patch-klemmeopptak fra vestibulære ganglion og spiral ganglionneuroner i det indre øret.

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Nevrovitenskap Somata Neonatal Gnagere Patch-klemme Recordings Inner Ear Ganglion Neurons Ion Channels Neurotransmitter Receptors Cell Diversity dissekere dissosierende dyrking Bipolar Neurons Kortsiktig Culturing Inner Ear Ear Somata Plating Spiral Ganglion Neurons Whole-cell Patch-clamp Recordings Perforert-patch Configuration Voltage-clamp Recordings Hyperpolarization-aktivert syklisk nukleotid-gated (HCN)-mediert Currents Ruptured-patch Configuration Cellular Prosesser G-proteinkoblede reseptorer
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