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Cancer Research

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JoVE Journal Cancer Research
Kwantificering van replicatiestress in eierstokkankercellen met behulp van enkelstrengs DNA-immunofluorescentie
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Kwantificering van replicatiestress in eierstokkankercellen met behulp van enkelstrengs DNA-immunofluorescentie

Article DOI: 10.3791/64920-v • 06:25 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1
1Division of Oncology, Department of Medicine, Siteman Cancer Center, Washington University School of Medicine

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Hier beschrijven we een op immunofluorescentie gebaseerde methode om de niveaus van enkelstrengs DNA in cellen te kwantificeren. Deze efficiënte en reproduceerbare methode kan worden gebruikt om replicatiestress te onderzoeken, een gemeenschappelijk kenmerk bij verschillende eierstokkankers. Bovendien is deze test compatibel met een geautomatiseerde analysepijplijn, wat de efficiëntie verder verhoogt.

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Cancer Research Nummer 192
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