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JoVE Journal Cancer Research
Kvantifiering av replikationsstress i äggstockscancerceller med hjälp av enkelsträngad DNA-immunofluorescens
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Kvantifiering av replikationsstress i äggstockscancerceller med hjälp av enkelsträngad DNA-immunofluorescens

Article DOI: 10.3791/64920-v • 06:25 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1
1Division of Oncology, Department of Medicine, Siteman Cancer Center, Washington University School of Medicine

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Här beskriver vi en immunofluorescensbaserad metod för att kvantifiera nivåerna av enkelsträngat DNA i celler. Denna effektiva och reproducerbara metod kan användas för att undersöka replikationsstress, ett vanligt inslag i flera äggstockscancerformer. Dessutom är denna analys kompatibel med en automatiserad analyspipeline, vilket ytterligare ökar dess effektivitet.

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Cancerforskning nummer 192
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