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Journal / Biochemistry / Un análisis extraíble cuantitativo optimizado de…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Un análisis extraíble cuantitativo optimizado de proteínas de unión al ARN utilizando ARN biotinilado corto
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Un análisis extraíble cuantitativo optimizado de proteínas de unión al ARN utilizando ARN biotinilado corto

Article DOI: 10.3791/64923-v • 07:55 min • February 17th, 2023
February 17th, 2023 •
1, 2, 2, 1,3, 1
1Center for Human Technology, Italian Institute of Technology (IIT), 2Department of Experimental Oncology, European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome

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Aquí, presentamos un método in vitro optimizado para descubrir, cuantificar y validar interactores de proteínas de secuencias específicas de ARN, utilizando extracto de proteína total de células humanas, perlas de estreptavidina recubiertas con ARN biotinilado y análisis de espectrometría de masas.

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Este mes en JoVE Número 192 Interacciones ARN-proteína extracción de proteínas proteínas de unión a ARN (RBP) ARN biotinilado aptámeros de ARN
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