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Journal / Immunology and Infection / Genereren van genetisch gemodificeerde Plasmodium …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Genereren van genetisch gemodificeerde Plasmodium berghei Sporozoïeten
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Genereren van genetisch gemodificeerde Plasmodium berghei Sporozoïeten

Article DOI: 10.3791/64992-v • 10:16 min • May 5th, 2023
May 5th, 2023 •
1, 1,2
1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

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Malaria wordt overgedragen door inoculatie van het sporozoietstadium van Plasmodium door geïnfecteerde muggen. Transgeen Plasmodium heeft ons in staat gesteld de biologie van malaria beter te begrijpen en heeft rechtstreeks bijgedragen aan de ontwikkeling van malariavaccins. Hier beschrijven we een gestroomlijnde methodologie om transgene Plasmodium berghei sporozoïeten te genereren.

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Genetisch gemodificeerde Plasmodium Berghei sporozoïeten malaria parasiet plasmodium anopheles-muggen ontwikkeling in het leverstadium klinische malaria immuunrespons transgene parasieten bloedstadium P. berghei speekselklieren van muggen in vivo experimenten in vitro experimenten groen fluorescerend eiwit (GFP) malaria in het leverstadium
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