Journal
/
Biology
/
Extensión de la vida útil de los neutrófilos conON-G y un ensayo de muerte espontánea in vitro…
JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content.
Extensión de la vida útil de los neutrófilos con CLON-G y un ensayo de muerte espontánea in vitro
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/65132-v
•
05:52 min
•
May 12th, 2023
Extensión de la vida útil de los neutrófilos con CLON-G y un ensayo de muerte espontánea in vitro
May 12th, 2023
•
Yuping Fan*1,2, Yan Teng*3, Fu tong Liu*4, Fengxia Ma1,2, Alan Y. Hsu5, Sizhou Feng1,2, Hongbo R. Luo5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Este protocolo detalla la preparación de CLON-G para extender la vida útil de los neutrófilos a más de 5 días y proporciona un procedimiento confiable para evaluar la muerte de neutrófilos con citometría de flujo y microscopía de fluorescencia confocal.