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Journal / Biology / CLON-G ile Nötrofil Yaşam Süresinin Uzatılması ve …
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CLON-G ile Nötrofil Yaşam Süresinin Uzatılması ve In Vitro Spontan Ölüm Testi
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CLON-G ile Nötrofil Yaşam Süresinin Uzatılması ve In Vitro Spontan Ölüm Testi

Article DOI: 10.3791/65132-v • 05:52 min • May 12th, 2023
May 12th, 2023 •
*1,2, *3, *4, 1,2, 5, 1,2, 5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center
* These authors contributed equally

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Bu protokol, nötrofil ömrünü 5 günden fazla uzatmak için CLON-G'nin hazırlanmasını detaylandırır ve akış sitometrisi ve konfokal floresan mikroskobu ile nötrofil ölümünü değerlendirmek için güvenilir bir prosedür sağlar.

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