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Immunology and Infection

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JoVE Journal Immunology and Infection
Enrichissement des vésicules extracellulaires natives et recombinantes de mycobactéries
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Enrichissement des vésicules extracellulaires natives et recombinantes de mycobactéries

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Ce protocole détaille l’enrichissement des vésicules extracellulaires mycobactériennes natives (mEVs) à partir de cultures axéniques de Mycobacterium smegmatis (Msm) et comment les MsmEVs recombinants contenant mCherry (un rapporteur fluorescent rouge) peuvent être conçus et enrichis. Enfin, il vérifie la nouvelle approche avec l’enrichissement de MsmEVs contenant la protéine EsxA de Mycobacterium tuberculosis.

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Immunologie et Infection Numéro 202 Vésicules extracellulaires vésicules recombinantes Esat-6 mycobactéries ExsA mCherry Cfp-29 bactéries
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