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Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

DOI:
10.3791/58949-v

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07:49 min

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January 07, 2019

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, , , , ,
1McGovern Medical School, Department of Microbiology and Molecular Genetics,University of Texas Health Science Center at Houston

Chapters

  • 00:04Title
  • 00:40Paramecia Growth and Maintenance and Bacterial Dose Determination
  • 02:23Paramecia Preparation for Food-borne Infection
  • 04:40Zebrafish Food-borne Infection and Pre-rate Determination
  • 06:30Results: Representative Zebrafish Bacterial Colonization Post Paramecia Consumption
  • 07:28Results

Summary

Automatic Translation

Automatic Translation

Zebrafish (Danio rerio) are becoming a widely-used vertebrate animal model for microbial colonization and pathogenesis. This protocol describes the use of the protozoan Paramecium caudatum as a vehicle for food-borne infection in zebrafish larvae. P. caudatum readily internalizes bacteria and get taken up by larval zebrafish through natural preying behavior.

Tags

Keywords: Paramecium CaudatumFood-borne InfectionsZebrafish LarvaeMicrobiologyGastrointestinal TractOral GavageE. Coli MG1655Colony Forming UnitsNon-ionic SurfactantPBSSelective PlatesBacterial ColoniesOptical DensityBacterial StainPhoto Bleaching

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