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Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Article DOI: 10.3791/945-v • 14:43 min • November 26th, 2008
November 26th, 2008 •
1, 1, 1, 1
1Research and Development, GE Healthcare Bio-Sciences AB

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A simple and specific method was demonstrated for fluorescent labeling and enhanced detection of cell surface proteins without a fractionation step. Differential abundance in cell surface proteins was analyzed using two-dimensional (2-D) electrophoresis and Ettan™ DIGE technology.

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Selective Labelling Cell-surface Proteins CyDye DIGE Fluor Minimal Dyes Surface Proteins Intracellular Signaling Environmental Adaptation Drug Treatment Disease Pathogenesis Pathology Protein-protein Interactions Sensitive Methods Reliable Methods Cell Surface Proteins Detection 2-D Electrophoresis Biomarkers Detection Complex Protein Samples Differential Changes Low Abundance Proteins Fractionation Enrichment Hydrophobic Nature High Molecular Weight Proteins Intact Cells Protocol Specific Labeling CyDye DIGE Fluor Minimal Dyes
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