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14:43 min
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November 26, 2008
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Summary
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Un metodo semplice e specifico è stato dimostrato per la marcatura fluorescente e la rilevazione maggiore di proteine di superficie cellulare, senza un passo di frazionamento. Abbondanza differenziale di proteine della superficie cellulare è stato analizzato mediante elettroforesi bidimensionale (2-D) e Ettan ™ tecnologia DIGE.
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Cite this Article
Hagner-McWhirter, A., Winkvist, M., Bourin, S., Marouga, R. Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes. J. Vis. Exp. (21), e945, doi:10.3791/945 (2008).
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