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Intravenöse Mikroinjektionen mit Zebrabärblingembryonen zu Acute Kidney Injury Study
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Intravenöse Mikroinjektionen mit Zebrabärblingembryonen zu Acute Kidney Injury Study

Article DOI: 10.3791/2079-v • 15:56 min • August 4th, 2010
August 4th, 2010 •
1, 2, 3, 1
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

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Wir beschreiben eine Technik der Mikroinjektion der Aminoglykosid, Gentamicin, in 2 Tagen nach fetilization (dpf) Zebrafischlarven zu akutem Nierenversagen (AKI) zu induzieren. Wir beschreiben ein Verfahren zur whole mount Immunhistochemie, Kunststoff eingebettet und Schnitte mit Zebrabärblingembryonen der AKI-vermittelten Schaden zu visualisieren.

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Developmental Biology Ausgabe 42 intravenöse Mikroinjektion Zebrafisch Gentamicin akutes Nierenversagen
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