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Journal / Neuroscience / 在单个细胞中的线粒体DNA复制的可视化EDU信号放大…
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Neuroscience

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在单个细胞中的线粒体DNA复制的可视化EDU信号放大

Article DOI: 10.3791/2147-v • 09:37 min • November 15th, 2010
November 15th, 2010 •
1, 2, 3
1Michigan Research Community, Undergraduate Research Opportunity Program, University of Michigan, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan

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我们制定了一个敏感的技术标签在单个细胞中新合成的线粒体DNA（mtDNA），为了研究线粒体DNA的生物合成。该技术结合了一个酪胺信号放大（TSA）协议EDU纳入可视化的神经元亚细胞车厢内线粒体DNA的复制。

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神经科学杂志，45期，线粒体，线粒体DNA（mtDNA），5 - 乙炔- 2' -脱氧尿苷（EDU），标签，酪胺信号放大，线粒体DNA的生物合成，背根神经节
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