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Lineage Märkning av Zebrafish Celler med laser Uncagable Fluorescein Dextran
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Lineage Märkning av Zebrafish Celler med laser Uncagable Fluorescein Dextran

Article DOI: 10.3791/2672-v • 07:36 min • April 28th, 2011
April 28th, 2011 •
1, 2, 2, 1
1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

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Detta protokoll skisserar ett sätt att märka och spåra öde små grupper av celler zebrafisk embryon med hjälp av UV-uncaging i bur fluorescein, följt av hela berget immunolabeling att förstärka signalen från uncaged fluorescein.

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Utvecklingsbiologi zebrafisk härkomst märkning bur fluorescein transgen
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