संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Neuroscience
 

Summary

इस वीडियो में, हम asymmetrically बरकरार में संवेदी अंग पूर्वपुस्र्ष कोशिकाओं और epidermal कोशिकाओं के विभाजन का जीना सेल इमेजिंग के लिए एक विधि का वर्णन

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Zitserman, D., Roegiers, F. Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae. J. Vis. Exp. (51), e2706, doi:10.3791/2706 (2011).

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Abstract

Protocol

Discussion

Disclosures

ब्याज की कोई संघर्ष की घोषणा की.

Acknowledgements

Materials

Name Company Catalog Number Comments
Scotch double stick tape
Glass slide
18mmX18mm coverslip
Forceps
Whatman paper
Silicone vacuum grease Dow Corning
5 ml syringe
Pipetter
Confocal or epifluorescence microscope

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References

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  2. Roegiers, F., Younger-Shepherd, S., Jan, L. Y., Jan, Y. N. Two types of asymmetric divisions in the Drosophila sensory organ precursor cell lineage. Nat Cell Biol. 3, 58-67 (2001).
  3. Bellaiche, Y., Gho, M., Kaltschmidt, J. A., Brand, A. H., Schweisguth, F. Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division. Nat Cell Biol. 3, 50-57 (2001).
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