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Journal / Biology / Ice-Cap: A Method for Growing Arabidopsis and…
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Biology

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Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Article DOI: 10.3791/3280-v • 11:15 min • November 9th, 2011
November 9th, 2011 •
1, 2, 1, 1, 1
1Horticulture Department, University of Wisconsin-Madison, 2Department of Zoology, Oregon State University

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Summary
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The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.

Tags

Ice-Cap Method Arabidopsis Tomato Plants 96-well Plates High-throughput Genotyping Tissue Sample Bottleneck Genotyping Pipeline High-throughput Solution Seedlings Agar Growth Media DNA Extraction Rapid Freezing
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