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Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
DOI:

08:08 min

December 08, 2011

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Chapters

  • 00:05Title
  • 01:51Collecting Lymphatic Isolation
  • 03:36Ratiometric Measurement of [Ca2+]
  • 04:46Pressure Control Protocol
  • 05:51[Ca2+] in Isolated Lymphatics
  • 07:42Conclusion

Summary

Automatic Translation

We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.

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