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Quantitative Measurement of the Immune Response and Sleep in Drosophila
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Chapters
- 00:05Title
- 01:33Bacterial Injection of Drosophila
- 04:40Determining the Bacterial Load
- 06:26Measuring NFkB Activity in Infected Flies
- 09:51Representative Measurements of Sleep and Immune Function During a Bacterial Infection
- 11:50Conclusion
To understand a link between the immune response and behavior, we describe a method to measure locomotor behavior in Drosophila during bacterial infection as well as the ability of flies to mount an immune response by monitoring survival, bacterial load, and real-time activity of a key regulator of innate immunity, NFκB.
Tags
Quantitative MeasurementImmune ResponseSleepDrosophilaInfectionExcess SleepMammalsDrosophila MelanogasterRobust Immune SystemMultidisciplinary ApproachLocomotor BehaviorPathogenic Strain Of BacteriaDuration Of SurvivalImmune FunctionNFκBTranscription FactorInnate Immune ResponseBacterial ClearanceResistance To InfectionTolerance To Infection
