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Intravital Imaging of the Mouse Popliteal Lymph Node
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Intravital Imaging of the Mouse Popliteal Lymph Node
DOI:

08:41 min

February 08, 2012

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Chapters

  • 00:05Title
  • 01:13Mouse Holder Assembly
  • 01:58Mouse Preparation
  • 03:27Surgery
  • 04:552-Photon Imaging Acquisition
  • 05:55Migration Analysis of GFP+ Lymphocytes in the Popliteal Lymph Node
  • 08:15Conclusion

Summary

Automatic Translation

Recent advances in 2-photon microscopy have enabled real-time in situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.

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