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Neuroscience

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JoVE Journal Neuroscience
Fremstilling af
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
Journal (Neuroscience)
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
Journal (Neuroscience)
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
 
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Fremstilling af

Article DOI: 10.3791/4264-v • 08:27 min • October 15th, 2012
October 15th, 2012 •
1, 1
1School of Life Sciences, Arizona State University

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In situ patch clamp-optagelser anvendes til elektrofysiologisk karakterisering af neuroner i intakt kredsløb. I Drosophila genetisk model patch fastspænding er vanskelig, fordi de CNS er lille og omgivet af en robust kappe. Denne artikel beskriver procedure for at fjerne hylsteret og rene neuroner for efterfølgende patch clamp optagelser.

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Neuroscience Molecular Biology Cellular Biology anatomi fysiologi Patch clamp, Elektrofysiologi motoneuron neuron CNS
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