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Captura selectivo de 5-hydroxymethylcytosine a partir de DNA genómico
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Captura selectivo de 5-hydroxymethylcytosine a partir de DNA genómico

Article DOI: 10.3791/4441-v • 06:26 min • October 5th, 2012
October 5th, 2012 •
1, 2, 2, 1
1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

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Descrita é um processo de rotulagem em duas etapas usando β-glicosiltransferase (β-GT) para transferir uma azida de glucose para 5 HMC, seguido por química do clique para transferir de um ligante de biotina para o enriquecimento fácil e densidade independente. Este método de marcação eficiente e específico permite o enriquecimento de 5 HMC com fundo extremamente baixo e de alto rendimento de mapeamento epigenômico via sequenciamento de última geração.

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Genética Edição 68 Química Biofísica 5-Hydroxymethylcytosine química rotulagem DNA genômico de alto rendimento seqüenciamento
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