Samtidig Multicolor Imaging af biologiske strukturer med Fluorescence Fotoaktivering Lokalisering Microscopy

* These authors contributed equally
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Curthoys, N. M., Mlodzianoski, M. J., Kim, D., Hess, S. T. Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy. J. Vis. Exp. (82), e50680, doi:10.3791/50680 (2013).

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Abstract

Materials

Name Company Catalog Number Comments
LabTek II chambers Nunc
Fluorescent beads Invitrogen F-8801 Beads for calibration
Tetraspeck beads Invitrogen T-7279 Four color beads for calibration
Objective immersion oil Zeiss 518F Immersion oil for high NA objective (dependent on choice of objective)
HPLC water Fisher Scientific W5-4
Media ATCC 30-2003 Or Cellgro 10-090
Antibiotics GIBCO 15070-063
serum Thermo Scientific SH30087.03
Lipofectamine Invitrogen 52887
Optimem I GIBCO 11058-021
Trypsin MPBiomedicals 1689149
paraformaldehyde Fisher Scientific AA433689M CAUTION: Toxic

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References

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