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JoVE Journal Neuroscience
Primer hipokampal nöronlar kalsiyum fosfat transfeksiyonu
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Primer hipokampal nöronlar kalsiyum fosfat transfeksiyonu

Article DOI: 10.3791/50808-v • 08:22 min • November 12th, 2013
November 12th, 2013 •
*1, *1, 1, 1, 1
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University
* These authors contributed equally

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Kalsiyum fosfat çökeltme kültürlenmiş hücrelerin transfeksiyonu için uygun ve ekonomik bir yöntemdir. Optimizasyonu ile, primer nöronlar gibi sert-transfect hücreleri üzerinde bu yöntemi kullanmak mümkündür. Burada astroglial hücreleri ile kültürlenmiş hipokampal nöronlar kalsiyum fosfat transfeksiyonu için, detaylı bir protokol açıklar.

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Neuroscience Sayı 81 primer hipokampal kalsiyum fosfat transfeksiyonu Coculture astroglial hücreler DNA
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