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Journal / Neuroscience / Hirnschnitt Biotinylierung: Eine
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Hirnschnitt Biotinylierung
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Hirnschnitt Biotinylierung: Eine

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Neuronale Membrantransport steuert dynamisch die Plasmamembran Protein Verfügbarkeit und wirkt sich deutlich auf die Neurotransmission. Bis heute hat es sich herausfordern, um neuronale endozytischen Handel bei erwachsenen Nervenzellen messen. Hier beschreiben wir eine hochwirksame, quantitative Methode, um schnelle Veränderungen in der Oberflächenproteinexpression ex vivo in akuten Hirnschnitten messen.

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Neuroscience Ausgabe 86 Illegaler Handel Endozytose Internalisierung Biotinylierung Gehirn Nervenzellen Transporter Proteinkinase C
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