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Untersuchen proteasomalen Abbau in einem zellfreien System in Pflanzen
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Untersuchen proteasomalen Abbau in einem zellfreien System in Pflanzen

Article DOI: 10.3791/51293-v • 07:43 min • March 26th, 2014
March 26th, 2014 •
1, 1, 1
1Department of Biochemistry and Cell Biology, Stony Brook University, State University of New York

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Gezielte Proteinabbau stellt einen wichtigen Regulationsmechanismus für die Zellfunktion. Es tritt über eine konservierte Ubiquitin-Proteasom-Weg, der Polyubiquitinketten an das Zielprotein, die dienen dann als molekulare "Tags" für die 26S-Proteasom isst. Hier beschreiben wir eine einfache und zuverlässige zellfreien Assays für den proteasomalen Abbau von Proteinen.

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Biochemie Ubiquitin / Proteasom-System 26S-Proteasom Proteinabbau Proteasom-Inhibitor Western Blotting pflanzengenetischer Transformation
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