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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
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Biology
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JoVE Journal Biology
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

DOI:
10.3791/52117-v

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11:23 min

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December 22, 2014

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, , , , ,
1Department of Biology,San Diego State University, 2DOE Joint Genome Institute, 3Department of Molecular, Cellular and Developmental Biology,University of Colorado, 4Department of Pediatrics, School of Medicine,University of Colorado

Chapters

  • 00:05Title
  • 02:01Sample Collection and Pre-treatment
  • 04:11Generating Viral Metagenome and Viral-like Particle Enrichment and Purification
  • 07:12Generating Microbial Metagenome
  • 08:57Results: Generation of Viral and Microbial Metagenomes
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

Using the cystic fibrosis airway as an example, the manuscript presents a comprehensive workflow comprising a combination of metagenomic and metatranscriptomic approaches to characterize the microbial and viral communities in animal-associated samples.

Tags

Keywords: MetagenomicsMetatranscriptomicsHost-associated MicrobiomeCystic FibrosisSputumDNA ExtractionRNA ExtractionHigh-throughput SequencingMicrobial CommunityViral CommunityTaxonomyFunctional Analysis

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