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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

DOI:
10.3791/51937-v

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09:35 min

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November 29, 2014

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, ,
1Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine,The Technion-Israel Institute of Technology

Chapters

  • 00:05Title
  • 01:25Protein Sample and Buffer Preparation
  • 02:38Sensor Chip and SPR Instrument Setup
  • 03:58Experiment Cycles
  • 07:32Results: On Line Monitoring of Ligand Immobilization and Determination of Kinetic Rate Constants by Injections of Multiple Analyte Concentrations
  • 09:07Conclusion

Summary

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Surface Plasmon Resonance (SPR) is a label-free method for detecting bio-molecular interactions in real time. Herein, a protocol for a membrane protein:receptor interaction experiment is described, while discussing the pros and cons of the technique.

Tags

Keywords: Surface Plasmon ResonanceSPRProtein-protein InteractionsMembrane ProteinsReal-time MeasurementsLabel-freeKineticsAssociationDissociationSensogramABC TransporterSubstrate Binding Protein

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