Surface Plasmon Resonance (SPR) is a label-free method for detecting bio-molecular interactions in real time. Herein, a protocol for a membrane protein:receptor interaction experiment is described, while discussing the pros and cons of the technique.
Livnat Levanon, N., Vigonsky, E., Lewinson, O. Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR). J. Vis. Exp. (93), e51937, doi:10.3791/51937 (2014).