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Journal / Bioengineering / Lignine Neerregeling van
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Bioengineering

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JoVE Journal Bioengineering
Lignine Neerregeling van
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Journal (Bioengineering)
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Photoacoustic Cystography
Journal (Bioengineering)
Photoacoustic Cystography
 
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Lignine Neerregeling van

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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Een dubbelstrengs RNA interferentie (dsRNAi) techniek wordt toegepast om down-reguleren van de maïs cinnamoyl co-enzym A-reductase (ZmCCR1) gen tot lagere planten ligninegehalte. Lignine down-regulatie van de celwand wordt gevisualiseerd door microscopische analyses en gekwantificeerd door de Klason methode. Compositionele veranderingen in hemicellulose en cellulose geanalyseerd.

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Biotechniek , Cinnamoyl-CoA reductase (CCR) dsRNAi Klason lignine meting celwand koolhydraat analyse gaschromatografie (GC)
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