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A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents
DOI:

08:30 min

May 22, 2014

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Chapters

  • 00:05Title
  • 01:20Intubation
  • 03:24Performing the Craniotomy
  • 04:25Two-photo Imaging
  • 06:22Results: Electroporation of Neural Tracers and Microvessel Permeability
  • 08:09Conclusion

Summary

Automatic Translation

A surgical method is described to expose the ventral skull in neonate rats. Using this approach it is possible to open a craniotomy to perform acute electrophysiology and two-photon microscopy experiments in the brainstem of anesthetized pups.

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