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Bio-lags Interferometry til måling Kinetik af protein-protein interaktioner og allosteriske Ligand Effects
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Bio-lags Interferometry til måling Kinetik af protein-protein interaktioner og allosteriske Ligand Effects

Article DOI: 10.3791/51383-v • 13:57 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

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Protokollerne her beskriver kinetiske analyser af protein-protein interaktioner med Bio-lags Interferometry. F-typen ATP syntase, som er involveret i cellulære energi metabolisme, kan hæmmes af sin ε underenhed i bakterier. Vi har tilpasset Bio-lags interferometri at studere interaktioner af det katalytiske kompleks med ε hæmmende C-terminale domæne.

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Kemi ATP syntase Bio-Layer Interferometry ligandinducerede konformationsændring Biomolekylær Interaction Analysis Allosterisk regulering Enzyme hæmning
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