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La reconstitución de la degradación de β-catenina en
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La reconstitución de la degradación de β-catenina en

Article DOI: 10.3791/51425-v • 09:41 min • June 17th, 2014
June 17th, 2014 •
*1, *1, 2, 1,3
1Department of Cell and Developmental Biology and Program in Developmental Biology, Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine
* These authors contributed equally

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Se describe un método para el análisis de la degradación de proteínas usando radiomarcado y proteínas de fusión luciferasa-en extracto de huevo de Xenopus y su adaptación para la selección de alto rendimiento para moduladores de moléculas pequeñas de la degradación de proteínas.

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Biología Molecular Número 88, la degradación de proteínas marca radiactiva luciferasa autorradiografía cribado de alto rendimiento
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