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Sekvens-specifik mærkning af nukleinsyrer og proteiner med methyltransferaser og cofaktor Analoger
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Sekvens-specifik mærkning af nukleinsyrer og proteiner med methyltransferaser og cofaktor Analoger

Article DOI: 10.3791/52014-v • 12:07 min • November 22nd, 2014
November 22nd, 2014 •
*1, *1, 1, 1, 1
1Institute of Organic Chemistry, Department of Chemistry, RWTH Aachen University
* These authors contributed equally

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DNA og proteiner sekvens-specifikt mærket med affinitet eller fluorescerende reportergrupper hjælp af DNA eller protein methyltransferaser og syntetiske cofaktor analoger. Afhængigt af cofaktor specificitet enzymer, aziridinen eller dobbelt aktiverede cofaktor analoger er ansat for en- eller to-trins mærkning.

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Biokemi , AdoMet SAM aziridinen cofaktor dobbelt aktiveret cofaktor methyltransferase DNA methylering protein methylering biotin mærkning fluorescens mærkning smilende mTAG
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