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核酸，蛋白质与甲基转移和辅酶类似物序列特异性标签
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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
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核酸，蛋白质与甲基转移和辅酶类似物序列特异性标签

DOI:
10.3791/52014-v

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12:07 min

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November 22, 2014

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, , , ,
1Institute of Organic Chemistry, Department of Chemistry,RWTH Aachen University

Chapters

  • 00:05Title
  • 02:26One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
  • 05:07Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
  • 08:41Results: Gel Analyses for SMILing and mTAG Reactions
  • 11:04Conclusion

Summary

Automatic Translation

Automatic Translation

DNA和蛋白质序列特异性标记的亲和力，或使用DNA或蛋白质的甲基和合成的辅助因子的类似物的荧光报告基团。取决于酶，氮丙啶或双活化的辅助因子类似物的辅因子特异性的用于一个或两个步骤的标签。

Tags

MethyltransferasesS-Adenosyl-l-methionine (AdoMet Or SAM)DNA MethylationRNA MethylationProtein MethylationAziridine CofactorsSMILing DNAMTAGDouble-activated AdoMet AnaloguesHistone MethylationClick ChemistryDNA GenotypingMethylation Detection

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