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JoVE Journal Developmental Biology
Mess Protein Stabilität in Wohnzebrafischembryonen unter Einsatz von Fluoreszenz Decay Nach Photokonversion (FDAP)
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Mess Protein Stabilität in Wohnzebrafischembryonen unter Einsatz von Fluoreszenz Decay Nach Photokonversion (FDAP)

Article DOI: 10.3791/52266-v • 09:45 min • January 28th, 2015
January 28th, 2015 •
1, 2, 1, 2
1Department of Molecular and Cellular Biology, Harvard University, 2Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

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Protein-Spiegel in Zellen und Geweben werden oft dicht durch das Gleichgewicht der Proteinproduktion und Freigabe geregelt. Verwendung Fluoreszenzabklingzeit Nach Photokonversion (FDAP) können die Clearance-Kinetik von Proteinen experimentell in vivo gemessen werden.

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Entwicklungsbiologie Fluoreszenz Decay Nach Photokonversion (FDAP) Proteinstabilität Clearance-Rate die konfokale Mikroskopie Photokonvertierbare Protein Dendra2 embryo Zebrafisch
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