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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering og flowcytometrianalyse gliom-infiltrerende perifere mononukleære blodceller
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Isolering og flowcytometrianalyse gliom-infiltrerende perifere mononukleære blodceller

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Præsenteres her er en enkel fremgangsmåde til isolering og flowcytometrisk analyse af gliom-infiltrerende perifert blod mononukleære celler, der giver tidsafhængige kvantitative data om antallet og aktiveringsstatus af immunceller ind i tidlige hjernetumor mikromiljø.

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Immunologi gliom perifere mononukleære celler (PBMC'er) galectin-1 (gal-1) GL26-Cit-gal1i GL26-Cit-NT Gr-1 naturlige dræberceller (NK-celler)
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