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Journal / Immunology and Infection / Genom-dækkende analyse af HDAC-hæmmer-medieret…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Genom-dækkende analyse af HDAC-hæmmer-medieret graduering af MicroRNA og mRNAs i B celler inducerede undergår klasse-skifte DNA rekombination og Plasma Celledifferentiering
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Journal (Immunology and Infection)
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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Journal (Immunology and Infection)
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
 
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Genom-dækkende analyse af HDAC-hæmmer-medieret graduering af MicroRNA og mRNAs i B celler inducerede undergår klasse-skifte DNA rekombination og Plasma Celledifferentiering

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetiske faktorer kan interagere med genetiske programmer til at modulere genekspression og regulere B-cellefunktion. Ved at kombinere in vitro B-celle stimulation, qRT-PCR, og høj overførselshastighed mikroRNA-sekvens og mRNA-sekvensen tilgange, kan vi analysere den epigenetiske graduering af miRNA og gen udtryk i B-celler.

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Immunologi sag 127 B-celle klasse-skifte DNA rekombination flowcytometri Histon deacetylase (HDAC) hæmmere (HDIs) mRNA mikroRNA mRNA-FF. miRNA-FF. plasma Celledifferentiering
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