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Immunology and Infection

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JoVE Journal Immunology and Infection
Genomet-omfattende analyse av HDAC Inhibitor-mediert modulering av microRNAs og mRNAs i B celler indusert Undergo klasse-bryteren DNA rekombinasjon og plasmacelle differensiering
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Genomet-omfattende analyse av HDAC Inhibitor-mediert modulering av microRNAs og mRNAs i B celler indusert Undergo klasse-bryteren DNA rekombinasjon og plasmacelle differensiering

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetic faktorer kan samhandle med genetiske programmer å modulere genuttrykk og regulere B celle-funksjonen. Ved å kombinere i vitro B-celle stimulering, qRT PCR, og høy gjennomstrømming microRNA-sekvens og mRNA-sekvens tilnærminger, kan vi analysere epigenetic modulering av miRNA og gene uttrykk i B-celler.

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Immunologi problemet 127 B celle klasse-bryteren DNA rekombinasjon flowcytometri histone deacetylase (HDAC) hemmere (HDIs) mRNA microRNA mRNA-seq miRNA-seq plasma celledifferensiering
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