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JoVE Journal Developmental Biology
Effektiv Isolering av funksjonelle holmer fra Neonatal Mouse Pancreas
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Effektiv Isolering av funksjonelle holmer fra Neonatal Mouse Pancreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

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Vi beskriver en protokoll her for å isolere intakt holmer fra neonatale mus. Pankreata ble delvis spaltet med kollagenase, fulgt av vasking og håndplukking. 20 - 80 islet klynger kan fås per bukspyttkjertel fra nyfødte mus, som er egnet for flere studier holmen.

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Developmental Biology transplantasjon kollagenase diabetes modning holmer insulin betaceller sekresjon neonatal håndplukking
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