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JoVE Journal Neuroscience
Hurtig og Raffineret CD11b Magnetisk Isolering af Primary Mikroglia med Enhanced Renhed og Alsidighed
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Hurtig og Raffineret CD11b Magnetisk Isolering af Primary Mikroglia med Enhanced Renhed og Alsidighed

Article DOI: 10.3791/55364-v • 07:54 min • April 13th, 2017
April 13th, 2017 •
*1, *1, 1, 1, 1, 1, 1, 1
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology, Iowa State University
* These authors contributed equally

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Her præsenteres en protokol til isolering mikroglia fra postnatal museunger (dag 1) til in vitro forsøg. Denne improviserede fremgangsmåde til isolation genererer både højt udbytte og renhed, en betydelig fordel i forhold alternative fremgangsmåder, der tillader essentielle eksperimenter med henblik på at belyse mikroglial biologi.

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Neuroscience CD11b mikroglia primær kultur magnetisk isolation hjerne aldring neurodegeneration neuroinflammation neurodegenerative lidelser
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