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Journal / Genetics / Kontrollerbar jonkanalen uttryck genom…
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Genetics

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JoVE Journal Genetics
Kontrollerbar jonkanalen uttryck genom inducerbara Transient transfektion
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Kontrollerbar jonkanalen uttryck genom inducerbara Transient transfektion

Article DOI: 10.3791/55370-v • 10:00 min • February 17th, 2017
February 17th, 2017 •
*1, *1, 1
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem (HUJI)
* These authors contributed equally

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Studera jonkanaler genom ett heterologt uttrycka systemet har blivit en kärntekniken i biomedicinsk forskning. I detta manuskript, presenterar vi en tidseffektiv metod för att uppnå hårt kontrollerad jonkanalen expression genom att utföra transient transfektion under kontroll av en inducerbar promotor.

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Genetik transfektion HEK293 Tet-repressor en kanal hela cellelektrofysiologi proteinuttryck inducerbart uttryck heterologa uttryck kalcium avbildning
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