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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation, karakterisering og rensning af makrofager fra væv ramt af fedme-relaterede Inflammation
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Journal (Immunology and Infection)
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
Journal (Immunology and Infection)
Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
 
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Isolation, karakterisering og rensning af makrofager fra væv ramt af fedme-relaterede Inflammation

Article DOI: 10.3791/55445-v • 07:46 min • April 3rd, 2017
April 3rd, 2017 •
1, 1, 2, 1, 1, 1, 1, 1
1Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences, Pennsylvania State University

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Denne protokol giver mulighed for en forsker at isolere og karakterisere væv-resident makrofager i forskellige hallmark betændt væv udvundet fra kost-induceret modeller af stofskiftesygdomme.

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Immunologi sag 122 fedme kost-induceret inflammation makrofager enzymatisk væv fordøjelse celle sortering enkelt cellesuspension monoklonale antistoffer FACS leveren perfusion aorta fordøjelse fedtvæv fordøjelsen
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