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Rensning af ekstracellulære Trypanosomes, herunder Afrika, fra blod af Anion-vekslere (Diethylaminoethyl-cellulose kolonner)
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

Rensning af ekstracellulære Trypanosomes, herunder Afrika, fra blod af Anion-vekslere (Diethylaminoethyl-cellulose kolonner)

DOI:
10.3791/58415-v

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14:26 min

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April 06, 2019

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, , , , , , , ,
1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux

Chapters

  • 00:00Title
  • 01:41Protocol
  • 09:25Results
  • 13:16Conclusion

Summary

Automatic Translation

Automatic Translation

Denne metode til trypanosome adskillelse fra blod afhænger deres overflade afgift er mindre negative end pattedyr blodlegemer. Inficeret blod er placeret og behandles på en anionbytteren kolonne. Denne metode, den mest passende diagnostiske for afrikanske trypanosomiasis, giver renset parasitter for immunologiske, biologiske, biokemiske, farmaceutiske og molekylærbiologiske undersøgelser.

Tags

African TrypanosomesSleeping SicknessAnimal African TrypanosomiasisTsetse FlyProtist ParasitesSerologyParasite Concentration TechniquesDEAE CelluloseAnion ExchangerCentrifugationMicroscopic ObservationDiagnosisPurified ParasitesImmunologicalBiologicalBiochemicalPharmaceuticalMolecular Biological InvestigationsPhosphate Buffered SalineGlucosePenicillinStreptomycinPhenol RedPHPotassium Phosphate

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