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Isolering og karakterisering af ekstracellulære vesikler produceret af jern begrænsede mykobakterier
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Isolering og karakterisering af ekstracellulære vesikler produceret af jern begrænsede mykobakterier

English

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6,966 Views

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08:27 min

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October 31, 2019

DOI:

10.3791/60359-v

DOI:
10.3791/60359-v

•

08:27 min
October 31, 2019

•

17 Views
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1Public Health Research Institute, New Jersey Medical School,Rutgers, The State University of New Jersey

Summary

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Mycobacterium tuberkulose viser øget produktion og frigivelse af ekstracellulære vesikler som reaktion på lave jern forhold. Dette arbejde beskriver en protokol til generering af lave jern forhold og metoder til rensning og karakterisering af mykobakterielle ekstracellulære vesikler frigivet som reaktion på jernmangel.

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Cite this Article

Gupta, S., Marcela Rodriguez, G. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (152), e60359, doi:10.3791/60359 (2019).

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