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Biochemistry

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JoVE Journal Biochemistry
Rekombinante Protein Expression, Kristallisation und biophysikalische Studien von a
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Journal (Biochemistry)
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Journal (Biochemistry)
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
 
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Rekombinante Protein Expression, Kristallisation und biophysikalische Studien von a

Article DOI: 10.3791/55576-v • 12:23 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1
1Division of Biological Science and Technology, Yonsei University

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Bacillus anthracis ist der obligatorische Erreger der tödlichen Inhalations-Milzbrand, so dass Studien über seine Gene und Proteine ​​streng reguliert sind. Ein alternativer Ansatz ist es, orthologe Gene zu untersuchen. Wir beschreiben biophysikochemische Studien eines B. anthracis ortholog in B. cereus , bc1531, die minimale experimentelle Ausrüstung erfordern und keine ernsthaften Sicherheitsbedenken haben.

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Biochemie Ausgabe 123, Rekombinantes Protein Kristallisation MazG Nukleotidpyrophosphohydrolase
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