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JoVE Journal Neuroscience
Isolamento e cultura de neurônios da medula espinhal de ratos neonatais
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Isolamento e cultura de neurônios da medula espinhal de ratos neonatais

Article DOI: 10.3791/55856-v • 07:49 min • July 11th, 2017
July 11th, 2017 •
1, 1, 1, 1
1Department of Surgery, Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus

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Este estudo apresenta uma técnica para o isolamento de neurônios de ratos neonatais WT. Requer a dissecção cuidadosa da medula espinhal do rato neonatal, seguida da separação de neurônios do tecido da medula espinhal através da clivagem mecânica e enzimática.

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Neurociência número 125 medula espinal neurônio do rato camundongos neonatais cultura celular purificação gradiente de densidade papaína NeuN MAP2
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