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Journal / Genetics / Chromatine Immunoprecipitatie (ChIP) in Muis…
JoVE Journal
Genetics

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JoVE Journal Genetics
Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen
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Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen

Article DOI: 10.3791/55907-v • 11:39 min • June 17th, 2017
June 17th, 2017 •
1, 1, 1
1Institute of Biochemistry, University of Giessen

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Dit werk beschrijft een protocol voor chromatine immunoprecipitatie (ChIP) met behulp van een volwassen T-cellijn van de muis. Dit protocol is geschikt om de verdeling van specifieke histonenmerken op specifieke promotor sites of genoombreed te onderzoeken.

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Genetica Uitgave 124 ChIP H3K4me1 H3K4me3 H3K27ac H3 histonenmerken sonicatie scheren chromatine regulering transcriptie.
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