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Bestemmelse af Optimal kromosomale placering for en DNA Element i Escherichia coli ved hjælp af en roman Transposon-medieret tilgang

Article DOI: 10.3791/55946-v • 11:12 min • September 11th, 2017
September 11th, 2017 •
1, 1, 2, 1
1Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, 2Department of Microbiology and Infection Control, Statens Serum Institut

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Her, blev en transposon-medieret tilfældige indsættelse af en ikke-kodende DNA element magt brugt til at løse sin optimale kromosomale holdning.

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Genetik sag 127 Random transposon indsættelse pool kultur konkurrence optimal genomisk kontekst hele-genome sequencing let gen walking celle cyklus analyse ved flowcytometri
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