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Bioengineering

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JoVE Journal Bioengineering
Kvantifisere mikroorganismer i lave konsentrasjoner bruker Digital holografiske mikroskopi (DHM)
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Kvantifisere mikroorganismer i lave konsentrasjoner bruker Digital holografiske mikroskopi (DHM)

Article DOI: 10.3791/56343-v • 07:27 min • November 1st, 2017
November 1st, 2017 •
1, 2, 3, 2, 1
1Department of Medical Engineering, California Institute of Technology, 2Department of Earth Sciences, University of Southern California, 3Jet Propulsion Laboratory, California Institute of Technology

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Digital holografiske mikroskopi (DHM) er en volumetriske teknikk som lar tenkelig prøver 50-100 X tykkere enn brightfield mikroskopi på sammenlignbare oppløsning, med fokusering utført etterbehandling. DHM brukes her til å identifisere, teller, og spore mikroorganismer på svært lav tetthet og sammenlignet med optisk densitet målinger, plate antall og direkte teller.

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